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Exprese proteinu NS5 viru klíšťové encefalitidy v lidských neurálních buňkách
JAKLOVÁ, Kateřina
This study focuses on the detection of tick-borne encephalitis virus (TBEV) NS5 protein in infected and NS5-transfected DAOY HTB-186 human neural cells. TBEV NS5 protein was shown to localize mainly on the membranes of the endoplasmic reticulum. An interesting finding was also nuclear localization, which is supported by the obtained data from both, confocal microscopy and subcellular fractionation.
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Patogeneze a klinické aspekty infekce virem klíšťové encefalitidy
ELSTEROVÁ, Jana
This thesis contributes to knowledge about the combined impacts of the pathogenesis of the tick-borne encephalitis virus (TBEV) and the immunopathogenesis of the host on the clinical course of acute tick-borne encephalitis (TBE). The thesis further focuses on the process of TBEV neuroinvasion and the utilization of the host's immune products as potential therapeutic interventions.
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Synthesis of new nucleosides as potential inhibitors of flaviviral replication
Horkelová, Simona ; Nencka, Radim (advisor) ; Janeba, Zlatko (referee)
Viruses of the Flaviviridae family are the causative agents of many dangerous diseases for which we currently have no known cure, and research into new drugs against them therefore represents one of the major challenges for modern medicinal chemistry. Targeting the proteins encoded by viruses is the most common approach to combat them. For flaviviruses, the non- structural protein NS5 exhibiting methyltransferase (MTase) and RNA-dependent-RNA polymerase (RdRp) enzyme activity appears to be one of the most suitable molecular targets. This bachelor thesis deals with the synthesis of new potential drugs capable of inhibition skill of flaviviral RdRp. C-nucleoside analogues were prepared, containing 2 types of heterocyclical base: 3-fluoropicolamide modified in positions 5 or 6 and pyrido[3,2-d]pyrimidine-4-amine modified in positions 6 or 7 using aryl or heteroaromatic substituent Key words: C-nucleosides, polymerase, flaviviruses, Tick-borne encephalitis virus, Suzuki reaction, Grignard reaction
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Antiviral vaccination - tick borne encephalitis virus
Solarová, Pavlína ; Drda Morávková, Alena (advisor) ; Janštová, Vanda (referee)
Tick borne encephalitis is by one of the most severe disease of central nervous system. Agent of this disease is tick-borne encephalitis virus, which is transmitted mainly by tick. Clinical picture of TBE is considerably different and varied in individuals - from quite infection without symptoms up to severe cerebro-spinal meningitis, in such cases attacked persons by this virus leave hospital with whole-life handicap. We can prevent this disease by inoculation. We can see it in the neighboring Austria, where TBE almost became extinct because 90% of population is vaccinated. Vaccines are commercially available from 1979. Nevertheless only 17% population is vaccinated against TBEV. The reason for it could be fact that insurance companies do not provide the vaccines as well as not very effective health education. One of possibilities how we can this tendency reverse would be higher awareness of pupils at schools. In school educational programs there is contained prevention of viral disease, especially in general biology, biology of viruses and education to health.
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Interakce viru klíšťové encefalitidy s cytoskeletem hostitelských buněk
PRANČLOVÁ, Veronika
This thesis is focused on the role of host cytoskeleton, primarily microtubules and microfilaments, during tick-borne encephalitis virus infection in human neuroblastoma cell line SK-N-SH and tick cell line IRE/CTVM19. The importance of cytoskeletal integrity and dynamics to the viral replication cycle were examined using specific chemical inhibitors showing the virus utilizes studied structures in both cell lines. Immunofluorescence microscopy revealed structural changes in the actin cytoskeleton during late infection in SK-N-SH cells. Moreover, differences in expression of cytoskeleton-associated genes in both cell lines were compared. Several genes with up-regulated expression in SK-N-SH cells were identified during late infection.
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